Electrophoretic Analysis of Excretory/secretory Antigens of Gastrothylax Crumenifer from Buffalo
نویسنده
چکیده
Live adult Gastrothylax crumenifer worms were collected from buffaloes slaughtered at local abattoirs and washed several times with PBS (pH 7.4). Live intact adult flukes were suspended in DPBS (pH 7.2) and incubated at 37°C in a BOD incubator for 8 hrs. The fluid was centrifuged at 7000 rpm for 30 min at 4°C and the supernatant was designated Excretory and Secretory (E/S) antigens. On Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis, seven major bands viz., 240, 220, 180,130, 92, 72 and 30 kDa and three minor bands having molecular weight (MW) lesser than 30 kDa size were observed.
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